Caprion

Accelerating precision medicine™

Cellular Enumeration

ImmuneCarta uses multiparametric flow cytometry and fluorescent beads to enumerate cell subsets such as CD34+ hematopoietic stem cells and T, B, and NK cell subsets. Critical features of our cellular enumeration service include:

  • Measurement of the frequency and absolute counts of lymphocyte subsets (T cells, B cells and NK cells) in whole blood samples
  • Validation based on guidelines which have been set by the International Society of Hematotherapy and Graft Engineering (ISHAGE)
  • Cellular enumeration panels are customized at ImmuneCarta to provide frequencies and absolute counts for a variety of cells and samples of interest
  • Absolute number of cells per uL of blood by comparing the number of cellular events to the number of bead events acquired by the cytometer
  • Percentages and absolute counts of CD34+ cells in whole blood after mobilization using hematopoietic growth factors

Benefits and Applications

  • Single platform considerably reduces sample processing time, making this assay cost-effective for the client
  • Accurate and reproducible enumeration of cells while following international guidelines (ISHAGE)
  • Proven track record-Involved in a CD34 proficiency testing program (UK NEQAS: United Kingdom National External Quality Assessment Service)

Immune response monitoring using flow cytometry to enumerate cells
Representative dot plot of CD34+ cells from a patient treated with a hematopoietic growth factor.